If you intend to change permissions on IIS related directories, you must grant the anonymous user, IUSR_<ComputerName>, the following:
Directory | Permissions |
Inetpub\Wwwroot |
READ (RX) |
%SystemRoot% |
READ (RX) |
%SystemRoot%\System32 |
READ (RX) |
%SystemRoot%\System32\Inetsrv |
READ (RX) |
%SystemRoot%\System32\Inetsrv\Asp (and all subdirectories) |
READ (RX) |
<Drive:>\Program Files\Common Files (and all subdirectories) |
READ (RX) |
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